TrueAdvance, whole genome amplification service

TrueAdvance is Expedeon’s services for whole genome amplification (WGA), with optional next generation sequencing (NGS) and/or data analysis, for single-cells, circulating tumor DNA or purified gDNA. Amplifying whole genomes without using random primers: TruePrime™ is based on multiple displacement amplification, but unlike any other WGA methods, it doesn’t rely on random primers. Instead, TruePrime™ uses the novel TthPrimPol DNA primase. Consequently, TruePrime™ doesn’t suffer from the usual amplification bias and primer artefacts, shows sensitivity down to femtograms of DNA, reduced allelic dropout (ADO), and is the only WGA method suitable for both analysis of single nucleotide variants (SNV) and copy number variants (CNV). Quickly checking genome coverage before sequencing: CovCheck™ is our unique quality control assay for amplified human samples. It is a PCR panel of 24 primers which can quickly determine genome coverage after amplification to screen samples suitable for further analysis.

Only charging for successful amplification results: We do not charge for samples that failed our CovCheck™ genome coverage quality control. Our prices per samples are fixed and disclosed, and depend on the total number of samples that pass CovCheck™. We charge a minimal and fixed prices depending on the total number of samples in any case.

Custom made genetic test design

Making Genetics offers the design and implementation of custom made genetic panels, including specific combination of PCR genetic variants of  interest for the costumer (celiac disease, sport performance, cardiovascular risk, obesity, diabetest type 2, metabolism, antioxidant capacity and others, consult availability).

Precision Medicine

NBD scientists have been involved in many national and international genomics initiatives, such as the sequencing of the tomato genome, genome-wide studies of mutations associated to chronic lymphocytic leukemia, the development of in silico tools for the annotation of mutations in cancer, genome-wide prediction of promoter regions, epigenetics processes associated to the packaging of DNA in nucleosomes and others.

Preparation of panels tailored for massive sequencing.

WG offers advisory specific services to doctors and hospitals for the design, development and manufacture of panels of specific profiles for sequencing.

Nutrigenomic services

Nutrigenomic services: as recognized experts in the nutrigenomic field, we offer among our services the desing of genetically personalized diets or the evidences of the molecular pathways involved in the effects of functional foods.

Specific tests for the diagnosis of the disease

For a specific diagnosis of the disease a comprehensive analysis of NGS data obtained by using bioinformatics tools and protocols validated at an international level by genomic experts, obtaining and differentiating, if necessary, acquired or somatic variants of those that are germ or inheritable. The genetic germ markers detected in the study communicate to the patient and his family as disease prevention and early diagnosis.

Procesamiento de muestras

Aislamiento de ácidos nucleicos (DNA, RNA, microRNAs) de tejidos fijado en formol y embebido en parafina, de tejido congelado, de sangre periférica y de fluidos biológicos. También se realizan aislamientos de proteínas de tejido congelado y de tejido fijado en formol y embebido en parafina

Interpretation of genome sequencing panels

A selection and interpretation of genetic data (structural variants, genetic mutations, and changes of expression) is clinically relevant, showing the genetic profile of the patient in the context of the approved personalized therapies (FDA, EMA) or latest phases of clinical trials. Finally, experts in the field of clinical genetics and/or oncology integrate data in a concise clinical report that communicates to clinicians.

Custom cloning.

As a highly expert company in cloning and expression, Canvax offers time-saving services for plasmids with different antibiotic markers, copy numbers, promoters, tags or fusions. Our team synthetize daily genes and sequences of any origin and sub-clone them in the most suitable customized-vector depending on your needs: constitutive or inducible expression, translation-transcriptional reporters, introduction of selectable markers for stable cell line generation, IP-free plasmid backbones or “de novo” constructions and much more. Some examples of the services that Canvax offers: (1) PCR cloning & subcloning; (2) Screening and transformation of E. coli; (3) Gene synthesis; (4) Minipreps and maxipreps; (5)DNA barcoding; (6) Restriction, purification and ligation of DNA; (7)Transcriptional and translational fusions; (8)Epitope tagging; (9) Competent cells production.

Identification of biomarkers based on genomic experiments

Making offers to identify biomarkers associated with the diagnostic and risk for a specific disease or drug response through of  differential gene expression, methylation analysis or association of polymorphisms.For this purpose we offer experimenal design, biological  (massive analysis and posterior validation) and bioinformatic analysis.