The chemical synthesis of peptides has been used for many years as an effective tool for both research and diagnostic purposes. In the past, liquid phase synthesis, or "Solution Phase Peptide Synthesis" was performed. However, advances in the knowledge of the chemical basis of the reaction have led to a synthesis of peptides using a solid phase, "Solid Phase Peptide Synthesis".
Development and production of recombinant proteins.
Bioingenium is a company dedicated to the development and production of recombinant proteins for R&D in biopharmaceutical, diagnostics and cosmetic industry. Bioingenium has a team of highly trained scientists with experience in microbial expression systems such as E. coli and Pichia pastoris and animal cells systems as HEK293 and CHO cells. Our expression systems have freedom to operate and our advantage is our strong knowledge in process development so we can bring your product from the laboratory bench to the industrial plant.
Aislamiento de ácidos nucleicos (DNA, RNA, microRNAs) de tejidos fijado en formol y embebido en parafina, de tejido congelado, de sangre periférica y de fluidos biológicos. También se realizan aislamientos de proteínas de tejido congelado y de tejido fijado en formol y embebido en parafina.
As a highly expert company in cloning and expression, Canvax offers time-saving services for plasmids with different antibiotic markers, copy numbers, promoters, tags or fusions. Our team synthetize daily genes and sequences of any origin and sub-clone them in the most suitable customized-vector depending on your needs: constitutive or inducible expression, translation-transcriptional reporters, introduction of selectable markers for stable cell line generation, IP-free plasmid backbones or “de novo” constructions and much more. Some examples of the services that Canvax offers: (1) PCR cloning & subcloning; (2) Screening and transformation of E. coli; (3) Gene synthesis; (4) Minipreps and maxipreps; (5)DNA barcoding; (6) Restriction, purification and ligation of DNA; (7)Transcriptional and translational fusions; (8)Epitope tagging; (9) Competent cells production.
Synthesis of families of peptides for studies of transportation in membranes: Design and synthesis of families of peptides that transport HIV in established lines. In vitro studies of cell infection.
Synthesis of families of peptides for studies of transportation in membranes: In vitro studies of cell infection.
Peptide synthesis for use in the development of antibodies (Polyclonal and Monoclonal): Design, synthesis, modifications and conjugation to carrier proteins for the utilisation of sequences which may be employed as a target for the development of antibodies, both polyclonal in different species (rabbit, chicken, mouse…) and monoclonal (mouse).
Peptide synthesis for in vivo studies. Design and synthesis of peptides for in vivo studies with animal models, murine, in the development of neurodegenerative diseases.
Design and synthesis of peptides for the development of diagnostic kits (IVD): Development of peptides for the study of diseases of great social impact- Studies of Coeliac Disease- Immunogenic sequences in gluten.
Thanks to our novel Recombinant Antibodies Technology, Canvax offers high quality Polyclonal and Monoclonal antibody Production services, in rabbit and mouse, or additional services like: (1) Protein A Antibody Affinity purification; (2) Peroxidase & Biotin coupling; (3) Functional validation in ELISA; (4) Western Blot; (5) Immunohistochemistry; (6)Flow cytometry services for analysis and sorting of cells. (7)Purification by protein G; (8) Antibody Isotyping and Characterization assays; (9) Liquid N2 storage (1 year); (10) Hybridoma cell culture and antibody purification; (11) Production and purification of scFvs, Fabs, vNARs or VHH.
Bacterial expression and purification: Expression of the enzyme D-xylose-dehydrogenasein E.coli (BL21), purification by molecular exclusion chromatography and characterisation of the enzymatic activity.
Expression and purification in mammalian cells: Transfection of CHO and HEK293 cell lines, expression and purification of membrane protein CD19 by affinity with a 6xHis tag. Analysis usingWB.
Development of a stable cell line: Construction of the target vector, transfection of cell line HEK293, validation of the expression of membrane protein CD34, using cytometry.
Monoclonal and polyclonal antibody development service: rabbit polyclonal antibodies
Development of rabbit polyclonal antibody for use ina kit based on "Lateral Flow Immunoassay": Development of tools against different pathogens (micro-organisms) for the fabrication of a rapid diagnosis kit. Application of antigens supplied by the client, whole inactivated cells.
Service for the development of rabbit polyclonal antibodies for use against human protein. No Ag supplied by the client: Study of antigenicity, antigen development based on the client's needs (WB), peptide synthesis and conjugation with transporting protein. Usual protocol, and testing done by applying the technique requested by the customer.
Service for the development of rabbit polyclonal antibodies against allergens: Polyclonal antibody development for measuring allergens tested by ELISA.
Service for the development of rabbit polyclonal antibodies against synthetic proteins supplied by clients: Polyclonal antibody development for the detection of native proteins in WB/IHC for research purposes.